Adenylate kinase 3(C22S) protein (His tag) (80R-1049)
Purified recombinant Human Adenylate kinase 3(C22S) protein
|Synonyms||Adenylate kinase 3, Adenylate kinase 3 alpha like 1 protein, AK6 protein, Adenylate kinase 3, RP11 6J24.4., Adenylate kinase 3 protein, Adenylate kinase-3 protein, AK3L1 protein, AKL3L1 protein, AK3 protein, AKL3L protein, Adenylate kinase-3, Adenylate kinase 3 protein, FIX protein|
Coomassie Blue stained SDS-PAGE of Adenylate kinase 3(C22S) protein (His tag) (80R-1049)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-223 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASK LLRAVILGPP GSGKGTVSQR IAQNFGLQHL SSGHFLRENI KASTEVGEMA KQYIEKSLLV PDHVITRLMM SELENRRGQH WLLDGFPRTL GQAEALDKIC EVDLVISLNI PFETLKDRLS RRWIHPPSGR VYNLDFNPPH VHGIDDVTGE PLVQQEDDKP EAVAARLRQY KDVAKPVIEL YKSRGVLHQF SGTETNKIWP YVYTLFSNKI TPIQSKEAY|
|Grade & Purity||> 90% pure|
|Molecular Weight||29.3 kDa (259 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 2mM DTT, 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Adenylate kinase (AK) is a ubiquitous monomeric enzyme involved in energy metabolism of prokaryotic and eukaryotic cells. Five isozymes of adenylate kinase have been identified in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell. Recombinant human AK3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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