AHSP protein (30R-1369)

Purified recombinant Human AHSP protein

Synonyms Erythroid associated factor protein, , Erythroid differentiation associated factor protein, Erythroid differentiation related factor protein, Alpha-hemoglobin stabilizing protein Alpha hemoglobin stabilizing protein protein, ERAF protein, EDRF protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of AHSP protein (30R-1369)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-102 amino acids: MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP SS
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 11.8 kDa (102aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance AHSP(Alpha-hemoglobin stabilizing protein), also known as ERAF(Erythroid associated factor), is an erythroid-specific protein that acts as a chaperone to prevent the aggregation of alpha-hemoglobin during normal erythroid cell development. It specifically protects free alpha-hemoglobin from precipitation in live cells and in solution. This protein is downregulated in transmissible spongiform encephalopathies (TSEs). It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Recombinant AHSP protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of AHSP protein (30R-1369) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £144.23
Size: 100 ug
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