AIF1 protein (His tag) (80R-1764)
Purified recombinant Human AIF1 protein
|Synonyms||IRT-1 protein, Allograft inflammatory factor 1 protein, AIF-1, IRT1 protein, AIF-1 protein, AIF-1 protein, AIF 1, AIF 1 protein, IBA1 protein, AIF1|
Coomassie Blue stained SDS-PAGE of AIF1 protein (His tag) (80R-1764)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-147 amino acids: MGSSHHHHHH SSGLVPRGSH MSQTRDLQGG KAFGLLKAQQ EERLDEINKQ FLDDPKYSSD EDLPSKLEGF KEKYMEFDLN GNGDIDIMSL KRMLEKLGVP KTHLELKKLI GEVSSGSGET FSYPDFLRMM LGKRSAILKM ILMYEEKARE KEKPTGPPAK KAISELP|
|Grade & Purity||> 95% pure|
|Molecular Weight||18.9 kDa (167aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 2mM DTT, 20% glycerol and 200mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||AIF1 (Allograft inflammatory factor 1) is induced by cytokines and interferon. This protein is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. AIF1 is a cytoplasm, calcium-binding protein whose expression in transplanted human hearts correlates with rejection and development of coronary artery vasculopathy (CAV). Recombinant human AIF1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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