AK2 protein (His tag) (80R-1079)
Purified recombinant Human AK2 protein
|Synonyms||AK 2, AK-2, AK 2 protein, Adenylate kinase isoenzyme 2 protein, AK-2 protein, EC 22.214.171.124, AK2, ADK2 protein, Adenylate kinase 2 protein, ATP AMP transphosphorylase protein, AK2 protein|
Coomassie Blue stained SDS-PAGE of AK2 protein (His tag) (80R-1079)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-239 amino acids: MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI|
|Grade & Purity||> 95% pure|
|Molecular Weight||28.6 kDa (259 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 7.5, containing 5mM DTT, and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||Adenylate kinase (AK) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Recombinant human AK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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