Alcohol Dehydrogenase antibody (20-AR03)

Rabbit polyclonal Alcohol Dehydrogenase antibody

Synonyms Polyclonal Alcohol Dehydrogenase antibody, Anti-Alcohol Dehydrogenase antibody, EC1.1.1.1 antibody, Alcohol dehydrogenase 1 antibody, YADH-1 antibody, Alcohol dehydrogenase I antibody
Specificity Saccharomyces cerevisiae ALDH
Cross Reactivity To be determined by end-user
Applications ELISA, IP, WB
Immunogen Alcohol dehydrogenase antibody was raised in rabbit using full length Alcohol Dehydrogenase isolated from yeast as the immunogen.

Images

Western Blot analysis using Alcohol Dehydrogenase antibody (20-AR03)

Western blot analysis with Alcohol Dehydrogenase antibody used to detect yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM) 800 labeled Goat-anti-Rabbit IgG [H + L] diluted 1:5, 000 for 45 min at room temperature. The IRDye(TM) 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Specifications

Host Rabbit
Method of Purification Alcohol Dehydrogenase antibody was purified by delipidation and defibrination.
Form & Buffer Monospecific antisera, absorbed, delipidated and defibrinated, supplied in 0.02M NaPO4, 0.15M NaCl, pH 7.2, with 0.01% NaN4

Usage & Assay Information

Usage Recommendations ELISA: 1:5,000-1:25,000, IP: 1:100, WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.

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Images

  • Western Blot analysis using Alcohol Dehydrogenase antibody (20-AR03) | Western blot analysis with Alcohol Dehydrogenase antibody used to detect yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM) 800 labeled Goat-anti-Rabbit IgG [H + L] diluted 1:5, 000 for 45 min at room temperature. The IRDye(TM) 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Availability: In stock

Price: £236.37
Size: 1 ml
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