AP1S2 protein (His tag) (80R-1842)
Purified recombinant AP1S2 protein
|Synonyms||DC22 protein, MRX59 protein, AP1S2, APS2 1, APS2-1 protein, APS2 1 protein, APS2-1, MGC:1902 protein, SIGMA1B protein, AP-1 complex subunit sigma-2 protein|
Coomassie Blue stained SDS-PAGE of AP1S2 protein (His tag) (80R-1842)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-157 amino acids: MGSSHHHHHH SSGLVPRGSH MQFMLLFSRQ GKLRLQKWYV PLSDKEKKKI TRELVQTVLA RKPKMCSFLE WRDLKIVYKR YASLYFCCAI EDQDNELITL EIIHRYVELL DKYFGSVCEL DIIFNFEKAY FILDEFLLGG EVQETSKKNV LKAIEQADLL QEEAETPRSV LEEIGLT|
|Grade & Purity||> 85% pure|
|Molecular Weight||20.7 kDa (177aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 40% glycerol, 0.1M NaCl and 2mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||AP1S2, also known as AP-1 complex subunit sigma-2, serves as the small subunit of AP-1 complex 1 and is a member of the adaptin protein family. Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. Recombinant human AP1S2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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