AP2A2 antibody (70R-9954)
Affinity purified rabbit polyclonal AP2A2 antibody
|Synonyms||Polyclonal AP2A2 antibody, Anti-AP2A2 antibody, HYPJ antibody, APA-2, ADTAB antibody, CLAPA2 antibody, HIP9 antibody, APA 2 antibody, APA-2 antibody, APA 2, adaptor-related protein complex 2, alpha 2 subunit antibody, AP2A2|
|Immunogen||AP2A2 antibody was raised in rabbit using the C terminal of AP2A2 as the immunogen|
|Assay Information||AP2A2 Blocking Peptide, catalog no. 33R-8256, is also available for use as a blocking control in assays to test for specificity of this AP2A2 antibody|
Western Blot analysis using AP2A2 antibody (70R-9954)
Western Blot showing AP2A2 antibody used at a concentration of 1-2 ug/ml to detect its target protein.
|Form & Buffer||Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.|
Usage & Assay Information
|Usage Recommendations||WB: 0.2-1 ug/ml|
Storage & Safety
|Storage||Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles.|
|Biological Significance||AP2A2 is a component of the adaptor protein complex 2 (AP-2). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif .|
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