Avidin protein (30C-CE0101A)
Purified Avidin protein from hen egg white
|Protein Type||Binding Protein|
SDS-PAGE analysis of Avidin protein (30C-CE0101A)
Lane 1: Avidin - reduced. Lane 2: Avidin - non-reduced. Load: 1.0 ug per lane. Predicted/Observed size: 16 kDa, 16 kDa for Avidin.
|Source||Chicken egg white|
|Method of Purification||Avidin protein was chromatographically purified.|
|Form & Buffer||Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamicin supfate. Immunoglobulin and Protease free. DO NOT add NaN3.|
Storage & Safety
|Storage||Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.|
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