Bcl-2 antibody (10R-1187)

Mouse monoclonal Bcl-2 antibody

Synonyms Monoclonal Bcl-2 antibody, Anti-Bcl-2 antibody, Oncogene B-cell leukemia 2. antibody, Bcl 2, B cell leukemia/lymphoma 2 antibody, B-cell lymphoma protein 2 alpha Apoptosis regulator Bcl 2 antibody, Bcl2 antibody, Bcl -2 antibody, B-cell lymphoma protein 2 alpha antibody, B-cell antibody, Bcl-2 antibody, C430015F12Rik antibody, Bcl2, Bcl 2 antibody, Bcl -2, 2 antibody, D630044D05Rik antibody, AW986256 antibody, Apoptosis regulator Bcl2 antibody, B cell CLL/lymphoma 2 antibody, D830018M01Rik antibody, Leukemia/lymphoma antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen Bcl-2 antibody was raised in mouse using recombinant human Bcl-2 (1-211aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of Bcl-2 antibody (10R-1187)

Cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Bcl-2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT1B5
Isotype IgG2b kappa
Source Anti-human Bcl2 antibody, clone AT1B5, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Bcl-2 protein.
Method of Purification Bcl-2 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:500, WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Bcl-2 (B-cell lymphoma protein 2 alpha) is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apoptosis. Bcl-2 protein has anti-apoptotic functions through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

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Images

  • Western Blot analysis of Bcl-2 antibody (10R-1187) | Cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Bcl-2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
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