Bcl2 protein (His tag) (80R-1136)
Purified recombinant Human Bcl2 protein
|Synonyms||2 protein, B-cell protein, Bcl 2, Bcl 2 protein, Leukemia/lymphoma protein, Bcl-2 protein, C430015F12Rik protein, AW986256 protein, B-cell lymphoma protein 2 alpha protein, Bcl2 protein, Bcl-2 protein, B cell CLL/lymphoma 2 protein, B-cell lymphoma protein 2 alpha Apoptosis regulator Bcl 2 protein, Apoptosis regulator Bcl2 protein, Bcl2, D630044D05Rik protein, Oncogene B-cell leukemia 2., B cell leukemia/lymphoma 2 protein, Bcl-2, D830018M01Rik protein|
Coomassie Blue stained SDS-PAGE of Bcl2 protein (His tag) (80R-1136)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-211 amino acids: MGSSHHHHHH SSGLVPRGSH MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D|
|Grade & Purity||> 90% pure|
|Molecular Weight||25.4 kDa (231 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, and 2 mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Bcl-2, also known as B-cell lymphoma protein 2 alpha, is an anti-apoptotic protein located primarily in the outer mitochondrial membrane that blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apotosis. Bcl-2 exerts its anti-apoptotic function through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). The Bcl-2 gene has been related with a number of cancers, including melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity. Recombinant Bcl-2 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.|
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