beta Tubulin antibody (10R-1191)

Mouse monoclonal beta Tubulin antibody

Synonyms Monoclonal beta Tubulin antibody, Anti beta Tubulin antibody, Tubulin beta-2B antibody, Tubulin beta 2b antibody, MGC8685 antibody, DKFZp566F223 antibody, Tubulin beta-2B chain antibody, bA506K6.1 antibody, TUBB2B antibody, FLJ98847 antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IF, WB
Immunogen beta Tubulin antibody was raised in mouse using recombinant human beta-Tubulin (1-445aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of beta Tubulin antibody (10R-1191)

The extracts of Mouse brain (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT5B2
Isotype IgG2b kappa
Source Anti-human beta-Tubulin antibody, clone AT5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human beta-Tubulin protein.
Method of Purification beta Tubulin antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:1,000, WB: 1:250-1:1,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

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Images

  • Western Blot analysis of beta Tubulin antibody (10R-1191) | The extracts of Mouse brain (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
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