BHMT protein (His tag) (80R-1013)
Purified recombinant Human BHMT protein
|Synonyms||EC 22.214.171.124 protein, Betaine homocysteine S methyltransferase protein, Betaine--homocysteine S-methyltransferase 1 protein, , BHMT protein, BHMT1 protein, Betaine homocysteine S methyltransferase 1 protein, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase protein, BHMT (Betaine-homocysteine methyltransferase) protein|
Coomassie Blue stained SDS-PAGE of BHMT protein (His tag) (80R-1013)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP VGGKKAKKGI LERLNAGEIV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFID LPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPP ASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM QQKEATTEQQ LKELFEKQKF KSQ|
|Grade & Purity||> 95% pure|
|Molecular Weight||49.2 kDa (443 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 7.5, containing 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis. Recombinant human BHMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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