Biotin reagent (Glucose Oxidase) (65C-CE0203)

Glucose Oxidase conjugated biotin labelling reagent

Protein Type Synthetic
Applications ELISA, IHC, WB

Specifications

Method of Purification Biotin reagent (Glucose Oxidase) was purified by ion exchange chromatography followed by dialysis.
Tag/Conjugate Glucose Oxidase
Form & Buffer Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl.

Usage & Assay Information

Usage Recommendations ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.

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Availability: In stock

Price: £140.22
Size: 5 mg
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