BMF protein (T7 tag) (80R-1154)
Purified recombinant Human BMF protein (T7 tag)
|Synonyms||Bcl2 modifying factor protein, FLJ00065., BMF protein, isoform 3 protein, Bcl2 modifying factor protein, isoform 3 Bcl 2 modifying factor protein|
Coomassie Blue stained SDS-PAGE of BMF protein (T7 tag) (80R-1154)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-129 amino acids: MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP|
|Grade & Purity||> 90% pure|
|Molecular Weight||15.6 kDa (144aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types. Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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