BMF protein (T7 tag) (80R-1154)

Purified recombinant Human BMF protein (T7 tag)

Synonyms Bcl2 modifying factor protein, FLJ00065., BMF protein, isoform 3 protein, Bcl2 modifying factor protein, isoform 3 Bcl 2 modifying factor protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of BMF protein (T7 tag) (80R-1154)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-129 amino acids: MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 15.6 kDa (144aa), confirmed by MALDI-TOF.
Tag/Conjugate T7 tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types. Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of BMF protein (T7 tag) (80R-1154) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £272.43
Size: 100 ug
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