BMP2 protein (30R-1250)
Purified recombinant Human BMP2 protein
|Synonyms||BMP-2, BMP2A protein, Bone Morphogenetic protein-2, BMP-2A protein, Bone morphogenetic Protein 2 BMP2 protein, Bone morphogenetic Protein 2 protein, BMP 2, BMP 2 protein, BMP-2 protein, BMP2|
Coomassie Blue stained SDS-PAGE of BMP2 protein (30R-1250)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||283-396 amino acids: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR|
|Grade & Purity||> 95% pure|
|Molecular Weight||13 kDa (115aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 10mM sodium citrate, pH 3.5, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||BMP-2 (Bone morphogenetic protein 2) is a multi-functional growth factor that belongs to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility. Recombinant human BMP-2 protein was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.|
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