BRCC36 antibody (10R-1156)

Mouse monoclonal BRCC36 antibody

Synonyms Monoclonal BRCC36 antibody, Anti-BRCC36 antibody, BRCC 36 antibody, BRCA1/BRCA2-containing complex subunit 3 antibody, BRCC36, BRCC 36, chromosome X open reading frame 53 antibody, C6.1A antibody, BRCC -36 antibody, BRCC -36
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, WB
Immunogen BRCC36 antibody was raised in mouse using recombinant BRCC36 (1-316aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of BRCC36 antibody (10R-1156)

Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT3B1
Isotype IgG1 kappa
Source Anti-human BRCC36 antibody, clone AT3B1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BRCC36 protein.
Method of Purification BRCC36 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance BRCC36 (BRCA1/BRCA2-containing complex, subunit 36) is a holoenzyme complex containing BRCA1, BRCA2. BRCC36 is an ubiquitin E3 ligase complex that has a role in DNA repair and enhances cellular survival following DNA damage. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint.

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Images

  • Western Blot analysis of BRCC36 antibody (10R-1156) | Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
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