CA5A antibody (70R-9981)
Affinity purified rabbit polyclonal CA5A antibody
|Synonyms||Polyclonal CA5A antibody, Anti-CA5A antibody, CAVA antibody, CAA 5 antibody, carbonic anhydrase VA, CA5 antibody, CAV antibody, CAA-5, CA5A, mitochondrial antibody, CAA 5, CAA-5 antibody|
|Immunogen||CA5A antibody was raised in rabbit using the C terminal of CA5A as the immunogen|
|Assay Information||CA5A Blocking Peptide, catalog no. 33R-1600, is also available for use as a blocking control in assays to test for specificity of this CA5A antibody|
Western Blot analysis using CA5A antibody (70R-9981)
Western Blot showing CA5A antibody used at a concentration of 1-2 ug/ml to detect its target protein.
|Form & Buffer||Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.|
Usage & Assay Information
|Usage Recommendations||WB: 0.2-1 ug/ml|
Storage & Safety
|Storage||Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles.|
|Biological Significance||Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2.|
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