Calf Intestinal Alkaline Phosphatase antibody (20C-CR2110RP)

Rabbit polyclonal Calf Intestinal Alkaline Phosphatase antibody

Synonyms Polyclonal Calf Intestinal Alkaline Phosphatase antibody, Anti-Calf Intestinal Alkaline Phosphatase antibody, ALPI antibody, IAP antibody, Alkaline phosphomonoesterase antibody, Glycerophosphatase antibody, Kasahara isozyme antibody, Intestinal alkaline phosphatase antibody
Specificity Bovine alkaline phosphotase
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Calf intestinal alkaline phosphatase antibody was raised in rabbit using alkaline phosphatase isoltaed from calf intestine as the immunogen.

Images

Western blot analysis using Calf Intestinal Alkaline Phosphatase antibody (200-4135)

Western Blot of rabbit anti Alkaline Phosphatase antibody Lane 1: Calf intestine Alkaline Phosphatase. Load: ~1.5 ug Block: 5% Blocking buffer 1 hour at 4 deg C. diluted to 1:1000 in 5% Blocking buffer overnight at 4 deg C. Goat anti Rabbit 611-103-122 Lot

Specifications

Host Rabbit
Method of Purification Calf Intestinal Alkaline Phosphatase antibody was purified by ion exchange chromatography followed by dialysis.
Form & Buffer IgG Fraction supplied as a liquid in PBS, pH 7.4, 0.01% NaN3.

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.

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Images

  • Western blot analysis using Calf Intestinal Alkaline Phosphatase antibody (200-4135) | Western Blot of rabbit anti Alkaline Phosphatase antibody Lane 1: Calf intestine Alkaline Phosphatase. Load: ~1.5 ug Block: 5% Blocking buffer 1 hour at 4 deg C. diluted to 1:1000 in 5% Blocking buffer overnight at 4 deg C. Goat anti Rabbit 611-103-122 Lot

Availability: In stock

Price: £84.13
Size: 1 ml
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