Carboxypeptidase Y antibody (20R-CR012)

Rabbit polyclonal Carboxypeptidase Y antibody

Synonyms Polyclonal Carboxypeptidase Y antibody, Anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, Carboxypeptidase Y antibody, CPY1 antibody, Vacuolar carboxypeptidase Y antibody, LBC1 antibody, PRC1 antibody, YMR297W antibody
Specificity Saccharomyces cerevisiae carboxypeptidase Y
Cross Reactivity To be determined by end-user
Applications ELISA, WB
Immunogen Carboxypeptidase Y antibody was raised in rabbit using Carboxypeptidase Y [Baker’s Yeast] as the immunogen.

Images

Western Blot analysis using Carboxypeptidase Y antibody (20R-CR012)

Both the antiserum and IgG fractions of anti-Carboxypeptidase Y are shown to detect under reducing conditions of SDS-PAGE the 61 KDa enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5, 000 dilution of the primary antibody is used followed by detection using Goat anti Rabbit IgG (H + L) (HRP) diluted 1:4, 000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Specifications

Host Rabbit
Method of Purification Carboxypeptidase Y antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form & Buffer 0.02M K3PO4, pH 7.2, with 0.15M NaCl and 0.01% NaN3.

Usage & Assay Information

Usage Recommendations ELISA: 10,000-1:42,000, WB: 1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance A carboxypeptidase is a protease enzyme that hydrolyzes (cleaves) the peptide bond of an amino acid residue at the carboxy-terminal (C-terminal) end. (Contrast with an aminopeptidase, which cleaves peptide bonds at the other end of the residue.) Humans, animals, and plants contain several types of carboxypeptidases which have diverse functions ranging from catabolism to protein maturation.

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Images

  • Western Blot analysis using Carboxypeptidase Y antibody (20R-CR012) | Both the antiserum and IgG fractions of anti-Carboxypeptidase Y are shown to detect under reducing conditions of SDS-PAGE the 61 KDa enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5, 000 dilution of the primary antibody is used followed by detection using Goat anti Rabbit IgG (H + L) (HRP) diluted 1:4, 000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Availability: In stock

Price: £460.72
Size: 100 ug
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