CD144 antibody (FITC) (60R-CR003ft)

Rabbit polyclonal CD144 antibody (FITC) conjugated

Synonyms Polyclonal CD144 antibody, Anti-CD144 antibody, CD-144, VE-Cadherin antibody, CD-144 antibody, CD 144, CD144, CD 144 antibody
Specificity Human
Cross Reactivity Bovine, mouse, rat
Applications FC
Immunogen CD144 antibody (FITC) was raised in rabbit using recombinant protein fragments corresponding to amino acids 1-258 of VE-cadherin as the immunogen.

Images

Flow cytometric assay using CD144 antibody (FITC) (60R-CR003ft)

Recognition of native VE-cadherin by anti-VE-Cad. Specificity of the anti-fragment antibody anti-VE-Cad was examined by flow cytometry using VE-cadherin-expressing CHO cells( A ) (hatched surface, VE-cadherin transfectant; open surface, CHO control), by immunofluorescence staining of fixed confluent endothelial cell monolayers (B ), or by Western blot using endothelial cell lysates ( C).

Specifications

Host Rabbit
Isotype IgG1
Method of Purification CD144 antibody (FITC) was purified by affinity chromatography.
Tag/Conjugate FITC
Form & Buffer Purified IgG supplied in TRIS buffered saline, pH7.4, with 1% BSA, and 0.02% NaN3.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations FC: Neat

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.

General Information

Biological Significance VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions.

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Images

  • Flow cytometric assay using CD144 antibody (FITC) (60R-CR003ft) | Recognition of native VE-cadherin by anti-VE-Cad. Specificity of the anti-fragment antibody anti-VE-Cad was examined by flow cytometry using VE-cadherin-expressing CHO cells( A ) (hatched surface, VE-cadherin transfectant; open surface, CHO control), by immunofluorescence staining of fixed confluent endothelial cell monolayers (B ), or by Western blot using endothelial cell lysates ( C).
  • Western blot analysis using CD144 antibody (FITC) (60R-CR003ft) | Immunoblot analysis of VE-cadherin and catenin expression in endothelial cells exposed to anti-Cad2 antibody. A, total cell lysates of untreated cells (lanes 1 and 4) and of cells treated with anti-Cad2 antibody for 2 h (lanes 2 and5) or 4 h (lanes 3 and6) were electrophoresed and transferred to nitrocellulose membranes before probing with anti-VE-Cad, anti-alpha-catenin, anti-beta-catenin, and anti-plakoglobin (plako) antibodies.Lanes 4–6 correspond to cells treated with cycloheximide for 4 h prior to the addition of anti-VE-Cad.B, shown are the results from the quantification of the amounts of VE-cadherin and catenins. The amounts of VE-cadherin (panel a), alpha-catenin (panel b), beta-catenin (panel c), and plakoglobin (panel d) were estimated, using image analysis software, from the ECL bands of the Western blots described for A. Shaded bars, without cycloheximide; white bars, with cycloheximide. The relative amounts

Availability: In stock

Price: £556.87
Size: 100 tests
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