CDA protein (His tag) (80R-1501)

Purified recombinant Human CDA protein

Synonyms CDD protein, Cytidine deaminase, Cytosine nucleoside deaminase protein, Cytidine aminohydrolase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of CDA protein (His tag) (80R-1501)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-146 amino acids: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 18.3 kDa(166aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl Buffer, pH 8.0, containing 1mM DTT, 2mM EDTA, 100mM NaCl, and 40% glycerol.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity:> 3.5 unit/mg. One unit of activity was defined as the amount required todeaminate 1.0 umole of cytidine per min at pH 7.5 at 25C

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of CDA protein (His tag) (80R-1501) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £236.37
Size: 100 ug
OR
Shipping
View Our Distributors