CHAF1B antibody (70R-1602)

Rabbit polyclonal CHAF1B antibody

Synonyms Polyclonal CHAF1B antibody, Anti-CHAF1B antibody, CAF-1 antibody, MPP7 antibody, CHAF1B, CHAFB-1 antibody, CHAFB 1, CHAFB 1 antibody, CAF1 antibody, MPHOSPH7 antibody, P60 antibody, CAF-IP60 antibody, CAF1P60 antibody, Chromatin Assembly Factor 1 Subunit B antibody, CAF1A antibody, CHAFB-1
Cross Reactivity Human
Applications IHC, WB
Immunogen CHAF1B antibody was raised using a synthetic peptide corresponding to a region with amino acids PPSSVPTSVISTPSTEEIQSETPGDAQGSPPELKRPRLDENKGGTESLDP
Assay Information CHAF1B Blocking Peptide, catalog no. 33R-7279, is also available for use as a blocking control in assays to test for specificity of this CHAF1B antibody

Images

Immunohistochemical staining using CHAF1B antibody (70R-1602)

CHAF1B antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml. Magnification is at 400X

Specifications

Host Rabbit
Method of Purification Total IgG Protein A purified
Molecular Weight 61 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 100ul distilled water for a 1mg/ml concentration of CHAF1B antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 5 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. CHAF1B corresponds to the p60 subunit and is required for chromatin assembly after replication. CHAF1B is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

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Images

  • Immunohistochemical staining using CHAF1B antibody (70R-1602) | CHAF1B antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml. Magnification is at 400X
  • Western Blot analysis using CHAF1B antibody (70R-1602) | CHAF1B antibody (70R-1602) used at 5 ug/ml to detect target protein.
  • Immunohistochemical staining using CHAF1B antibody (70R-1602) | CHAF1B antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of renal tubule (arrows) in Human Kidney. Magnification is at 400X

Availability: In stock

Price: £220.34
Size: 100 ug
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