Polyclonal Collagen Type VI antibody, Anti-Collagen Type VI antibody, CollagenVI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody
Specificity
Bovine/Human collagen type VI
Cross Reactivity
To be determined by end-user
Applications
ELISA, IHC, IP, WB
Immunogen
Collagen type VI antibody was raised in rabbit using collagen type VI from human and bovine placenta as the immunogen.
Immunohistochemical staining using Collagen Type VI antibody (biotin) (60R-CR006bt)
Anti collagen VI antibody (1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0. 01 M sodium citrate buffer, pH 6. 0 at 99-100 deg C - 20 minutes for antigen retrieval.
Collagen Type VI antibody (biotin)
Immunohistochemical staining using Collagen Type VI antibody (biotin) (60R-CR006bt)
Anti collagen VI antibody (1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0. 01 M sodium citrate buffer, pH 6. 0 at 99-100 deg C - 20 minutes for antigen retrieval.
Specifications
Host
Rabbit
Method of Purification
Collagen Type VI antibody (biotin) was purified by affinity chromatography.
Tag/Conjugate
Biotin
Form & Buffer
0.125M Na2B4O7, pH 8.0, 0.075M NaCl, 0.005M EDTA, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues
Storage & Safety
Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
General Information
Biological Significance
Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Collagen alpha-1(VI) chain is a protein that in humans is encoded by the COL6A1 gene. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively.
