CX3CL1/Fractalkine protein (His tag) (80R-1192)
Purified recombinant Human CX3CL1/Fractalkine protein
|Synonyms||CXCL1/Fractalkine-3 protein, ABCD-3 protein, NTT protein, CX3CL1 protein, CXC3C protein, CXCL1/Fractalkine 3, CXCL1/Fractalkine-3, CXCL1/Fractalkine 3 protein, CX3CL1/Fractalkine, neurotactin, neurotactin) protein, fractalkine protein, SCYD1 protein, NTN protein, small inducible cytokine subfamily D (Cys-X3-Cys) member 1 (fractalkine protein, CXC3 protein, C3Xkine protein, Chemokine (C-X3-C motif) ligand 1 protein|
Coomassie Blue stained SDS-PAGE of CX3CL1/Fractalkine protein (His tag) (80R-1192)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||25-100 amino acids: MGSSHHHHHH SSGLVPRGSH MQHHGVTKCN ITCSKMTSKI PVALLIHYQQ NQASCGKRAI ILETRQHRLF CADPKEQWVK DAMQHLDRQA AALTRNG|
|Grade & Purity||> 90% pure|
|Molecular Weight||10.9 kDa (97aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in PBS, pH 7.4, 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||CX3CL1 is also known as fractalkine. CX3CL1 has a unique CX3C cysteine motif near the amino terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin like stalk. It is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Also, CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Recombinant human CX3CL1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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