Diphtheria IgG ELISA Kit (55R-RE56191)

ELISA kit for detection of Diphtheria IgG in the research laboratory

Synonyms Diphtheria toxin IgG ELISA Kit
Applications ELISA
Assay Information The Diphtheria toxoid IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Diphtheria Toxoid antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Diphtheria Toxoid antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as pote

General Information

Biological Significance The etiologic agent of diphtheria is the Corynebacterium diphtheriae. These gram-positive bacteria prefer a microaerophilic to anaerobic environment. Its pathogenicity is based on the secretion of an exotoxin that is circulating in the blood and effecting the heart muscle, kidneys and CNS. The Diphtheria toxoid will be produced by lysogenic strains.

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Diphtheria IgG ELISA Kit Protocol

Added: Jun 22, 2015

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Availability: In stock

Price: £164.26
Size: 96 wells
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