Dnak protein (30R-1022)

Purified recombinant E.coli Dnak protein

Synonyms Heat shock 70 kDa protein protein, Chaperone protein dnaK protein, dnaK protein, Co chaperone with DnaJ protein, Dnak (C-term protein, , Heat shock protein 70 protein, Substrate binding domain protein, Chaperone Hsp 70 protein, HSP70 protein, Heat shock 70 kDa protein protein, 385-638) protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Dnak protein (30R-1022)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues Substrate binding domain, C-term; 385-638 amino acids: MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HSTRKQVEEA GDKLPADDKT AIESALTALE TALKGEDKAA IEAKMQELAQ VSQKLMEIAQ QQHAQQQTAG ADASANNAKD DDVVDAEFEE VKDKK
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 27.7 kDa (255aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 25mM Tris-HCl, pH 7.5, containing 100 mM NaCl, 5mM DTT, and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.

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Images

  • Coomassie Blue stained SDS-PAGE of Dnak protein (30R-1022) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £144.23
Size: 100 ug
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