DsbE protein (30R-1283)
Purified recombinant E.coli DsbE protein
|Synonyms||yejQ protein, Thiol:disulfide interchange protein dsbE Cytochrome c biogenesis protein ccmG..ECK2187 protein, Periplasmic thioredoxin of cytochrome c type biogenesis protein, CcmG protein, JW2183.|
Coomassie Blue stained SDS-PAGE of DsbE protein (30R-1283)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||26-185 amino acids: MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q|
|Grade & Purity||> 95% pure|
|Molecular Weight||18.1 kDa (161aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 2mM EDTA, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E. coli and purified by using conventional chromatography.|
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