eFIMC protein (His tag) (80R-1689)
Purified recombinant E.coli eFIMC protein
|Synonyms||ECK4307 protein, required for type 1 fimbriae protein, JW4279 protein, Periplasmic chaperone protein,|
Coomassie Blue stained SDS-PAGE of eFIMC protein (His tag) (80R-1689)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||37-241 amino acids: MGSSHHHHHH SSGLVPRGSH MGVALGATRV IYPAGQKQEQ LAVTNNDENS TYLIQSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLFWMNVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLINPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME|
|Grade & Purity||> 95% pure|
|Molecular Weight||25 kDa (226aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||The 23 kDa two-domain periplasmic chaperone FIMC from Escherichia coli is required for the assembly of type-1 pili, which are filamentous, highly oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages. Recombinant E. coli FIMC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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