EGF protein (30R-1056)

Purified recombinant Human EGF protein

Synonyms Epidermal growth factor protein, Urogastrone protein, Epidermal growth factor protein, epidermal growth factor (beta-urogastrone), EGF protein, Pro-epidermal growth factor protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: Balb-3T3 (Mus musculus (mouse)) Maintenance Condition: RPMI 1640 containing 10% FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation time: 24 hr (after sample treatment) Concentration range: 1 pg/ml - 1 ug/ml Detection method: BrdU assay

Specifications

Residues MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 6.3 kDa (54aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is < 0.89 ng/ml . Measured in a cell proliferation assay using mouse Balb3T3 cell.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Recombinant human epidermal growth factor(EGF) is a 6.3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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Availability: In stock

Price: £176.28
Size: 100 ug
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