EGFR antibody (10R-E101a)

Mouse monoclonal EGFR antibody

Synonyms Monoclonal EGFR antibody, Anti-EGFR antibody, Epidermal Growth Factor antibodyEgf receptor antibody, panitumumab antibody, epidermal growth factor receptor antibody, egfr antibody, v-erb-b homolog antibody, egf-r antibody, erbb1 antibody
Specificity Human
Cross Reactivity To be determined by end-user
Applications ICC, IHC, IP, WB
Immunogen EGFR antibody was raised in mouse using human A431 membrane protein as the immunogen.

Images

Immunocytochemistry staining using EGFR antibody (10R-E101a)

A431 cells were stained with 2ug/ml of anti-EGF Receptor (neutralizing), clone LA1, Alexa Fluor 488 conjugate (Panel A) or Alexa Fluor 488-conjugated Normal Mouse IgG (Panel B) (green) and DAPI (blue).

Specifications

Host Mouse
Clone LA1
Isotype IgG1
Method of Purification EGFR antibody was purified by Protein G chromatography.
Molecular Weight 180 kDa (predicted detection band MW)
Form & Buffer Lyophilized from 250ul 0.1M tris-glycine, pH 7.4, 0.15M NaCl.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze-thaw cycles.

General Information

Biological Significance The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGF alpha). EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity.

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Images

  • Immunocytochemistry staining using EGFR antibody (10R-E101a) | A431 cells were stained with 2ug/ml of anti-EGF Receptor (neutralizing), clone LA1, Alexa Fluor 488 conjugate (Panel A) or Alexa Fluor 488-conjugated Normal Mouse IgG (Panel B) (green) and DAPI (blue).
  • Western blot analysis using EGFR antibody (10R-E101a) | Western blot analysis of non-reduced A431 cell lysate probed with anti-EGF Receptor (1ug/ml)

Availability: In stock

Price: £500.58
Size: 250 ug
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