EGFR antibody (20R-ER004)

Rabbit polyclonal EGFR antibody

Synonyms Polyclonal EGFR antibody, Anti-EGFR antibody, egfr antibody, Epidermal Growth Factor antibodyEgf receptor antibody, egf-r antibody, v-erb-b homolog antibody, epidermal growth factor receptor antibody, panitumumab antibody, erbb1 antibody
Cross Reactivity To be determined by end-user
Applications ELISA, IP, WB
Immunogen EGFR antibody was raised in rabbit using a synthetic peptide corresponding to a region near the carboxy terminus which is identical in human, mouse and rat EGFR, as the immunogen.

Images

Western Blot analysis using EGFR antibody (20R-ER004)

Combined immunoprecipitation and western blot using EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15'' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 ul) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1’. Other detection systems will yield similar results.

Specifications

Host Rabbit
Form & Buffer Supplied as a liquid with 0.01% NaN3.
Concentration 85.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, IP: 2.5 ug/ml, WB: 1:1,000-1:10,000

Storage & Safety

Storage Store at 4 deg C. Dilute only prior to immediate use.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial to maintaining a normal balance in cell growth and development. Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors. For example, the epidermal growth factor receptor (EGFR) is mutated and/or over-expressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and ErbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis. EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4/neu. EGFR can heterodimerize with each of the members of this family.

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Images

  • Western Blot analysis using EGFR antibody (20R-ER004) | Combined immunoprecipitation and western blot using EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15'' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 ul) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1’. Other detection systems will yield similar results.

Availability: In stock

Price: £412.64
Size: 250 ul
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