EGLN1 antibody (10R-1165)

Mouse monoclonal EGLN1 antibody

Synonyms Monoclonal EGLN1 antibody, Anti-EGLN1 antibody, EGLN 1 antibody, PNAS-137 antibody, HIF prolyl hydroxylase 2 antibody, EGLN1, Prolyl hydroxylase domain-containing protein 2 antibody, EGLN -1 antibody, C1orf12 antibody, PHD2 antibody, egl nine homolog 1 (C. elegans) antibody, HIFPH2 antibody, HPH-2 antibody, EGLN 1, SM-20 antibody, EGLN -1, ZMYND6 antibody, HIF prolyl hydroxylase 2 antibody, SM20 antibody, HIF-PH2 antibody, DKFZp761F179 antibody, PNAS-118 antibody, ECYT3 antibody, Hypoxia-inducible factor prolyl hydroxylase 2 antibody, HPH2 antibody, EGL nine (C.elegans) homolog 1 antibody, HIF-prolyl hydroxylase 2 antibody, Egl nine homolog 1 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen EGLN1 antibody was raised in mouse using recombinant EGLN1 (271-426aa) purified from E. coli as the immunogen.

Images

Immunofluorescent staining using EGLN1 antibody (10R-1165)

HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).

Specifications

Host Mouse
Clone AT21E9
Isotype IgG2b kappa
Source Anti-human EGLN1 antibody, clone AT1E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EGLN1 protein.
Method of Purification EGLN1 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:500-1:1,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. The EGLN family, which are known to be cellular oxygen sensors, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.

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Images

  • Immunofluorescent staining using EGLN1 antibody (10R-1165) | HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
  • Western Blot analysis of EGLN1 antibody (10R-1165) | Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human EGLN1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

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Price: £212.33
Size: 100 ul
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