EIF1B protein (His tag) (80R-1721)
Purified recombinant Human EIF1B protein
|Synonyms||Eukaryotic translation initiation factor 1B protein, EIFB 1 protein, GC20 protein, EIFB-1, EIFB 1, EIF1B, EIFB-1 protein|
Coomassie Blue stained SDS-PAGE of EIF1B protein (His tag) (80R-1721)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-113 amino acids: MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF|
|Grade & Purity||> 95% pure|
|Molecular Weight||15.0 kDa (133aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||EIF1B is crucial for the scanning process in vitro. During the scanning process, EIF1B is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. This protein enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. Recombinant human EIF1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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