EIF4B antibody (70R-1418)

Rabbit polyclonal EIF4B antibody raised against the C terminal of EIF4B

Synonyms Polyclonal EIF4B antibody, Anti-EIF4B antibody, EIFB 4, EIF4B, EIFB-4 antibody, EIFB 4 antibody, Eukaryotic Translation Initiation Factor 4B antibody, EIFB-4
Specificity EIF4B antibody was raised against the C terminal of EIF4B
Cross Reactivity Human
Applications IHC, WB
Immunogen EIF4B antibody was raised using the C terminal of EIF4B corresponding to a region with amino acids NPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQ
Assay Information EIF4B Blocking Peptide, catalog no. 33R-6824, is also available for use as a blocking control in assays to test for specificity of this EIF4B antibody

Images

Immunohistochemical staining using EIF4B antibody (70R-1418)

EIF4B antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Myocardial cells (arrows) in Human Heart. Magnification is at 400X

Specifications

Host Rabbit
Method of Purification Total IgG Protein A purified
Molecular Weight 67 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 100ul distilled water for a 1mg/ml concentration of EIF4B antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1.25 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance IF4B contains 1 RRM (RNA recognition motif) domain and is required for the binding of mRNA to ribosomes. Functions of EIF4B are in close association with EEF4-F and EIF4-A. It binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP and promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

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Images

  • Immunohistochemical staining using EIF4B antibody (70R-1418) | EIF4B antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Myocardial cells (arrows) in Human Heart. Magnification is at 400X
  • Western Blot analysis using EIF4B antibody (70R-1418) | EIF4B antibody (70R-1418) used at 1.25 ug/ml to detect target protein.

Availability: In stock

Price: £220.34
Size: 100 ug
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