elF5A protein (30R-1272)
Purified recombinant Human elF5A protein
|Synonyms||MGC104255., MGC99547 protein, EIF5A1 protein, EIF 5A protein, uORF protein, uORF A protein, Eukaryotic initiation factor 5A isoform 1 protein, elFA 5, EIF-5A protein, eIF5AI protein, eIF4D protein, Eukaryotic translation initiation factor 5A protein, Eukaryotic translation initiation factor 5A 1 protein, elFA-5 protein, eIF 5A 1 protein, eIF 5A1 protein, elFA-5, elFA 5 protein, Rev binding factor protein, Eukaryotic translation initiation factor 5A isoform B eIF 4D protein, elF5A, Eukaryotic initiation factor 5A protein|
Coomassie Blue stained SDS-PAGE of elF5A protein (30R-1272)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-154 amino acids: MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK|
|Grade & Purity||> 95% pure|
|Molecular Weight||16.8 kDa (154 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 50mM Tris-HCl buffer, pH 7.5, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Eukaryotic translation initiation factor 5A (eIF5A) is the only protein known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer. Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques.|
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