EpCAM antibody (biotin) (61R-E110aBT)

Biotinylated Mouse monoclonal EpCAM antibody

Synonyms Monoclonal EpCAM antibody, Anti-EpCAM antibody, Epithelial Specific Antigen antibody, Ep-CAM antibody
Specificity Human
Cross Reactivity To be determined by end-user
Applications IHC-F, IHC-P
Immunogen EpCAM antibody was raised in mouse using small cell lung carcinoma cells as the immunogen.

Images

Immunohistochemical staining using EpCAM antibody (biotin) (61R-E110aBT)

Formalin-fixed, paraffin-embedded human breast cancer stained with EpCAM antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.

Specifications

Host Mouse
Clone VU-1D9
Isotype IgG1 kappa
Method of Purification EpCAM antibody was purified by Protein G chromatography.
Molecular Weight 40-43 kDa (predicted detection band MW)
Tag/Conjugate Biotin
Form & Buffer Antibody purified from ascites fluid by protein G chromotography and supplied in 10mM PBS, pH 7.4, with 0.2% BSA and .09% NaN3.
Concentration 0.2 mg/ml

Usage & Assay Information

Usage Recommendations IHC-F: 1-2 ug/ml, IHC-P: 1-2 ug/ml

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.

General Information

Biological Significance EGP40 is a 40 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. It reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

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Images

  • Immunohistochemical staining using EpCAM antibody (biotin) (61R-E110aBT) | Formalin-fixed, paraffin-embedded human breast cancer stained with EpCAM antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.

Availability: In stock

Price: £580.91
Size: 200 ug
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