EphA2 antibody (10R-1108)

Mouse monoclonal EphA2 antibody

Synonyms Monoclonal EphA2 antibody, Anti-EphA2 antibody, EphA 2 antibody, Ephrin receptor EphA2 antibody, epoxide hydrolase 1 microsomal (xenobiotic) antibody, EphA2, EphA -2, EphA 2, EphA -2 antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IHC, WB
Immunogen EphA2 antibody was raised in mouse using recombinant human EphA2 (559-976aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of EphA2 antibody (10R-1108)

The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human EphA2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 3D7
Isotype IgG2b kappa
Source Anti-human EphA2 antibody, clone 3D7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EphA2 protein.
Method of Purification EphA2 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:100-1:200, WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk). Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.

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Images

  • Western Blot analysis of EphA2 antibody (10R-1108) | The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human EphA2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
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