ERP44 protein (His tag) (80R-1440)
Purified recombinant Human ERP44 protein
|Synonyms||PDIA10 protein, ERP 44 protein, ER protein 44 protein, TXNDC4 protein, ERP-44 protein, Endoplasmic reticulum protein 44, ERP44, ERP 44, ERP-44|
Coomassie Blue stained SDS-PAGE of ERP44 protein (His tag) (80R-1440)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||30-406 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMEI TSLDTENIDE ILNNADVALV NFYADWCRFS QMLHPIFEEA SDVIKEEFPN ENQVVFARVD CDQHSDIAQR YRISKYPTLK LFRNGMMMKR EYRGQRSVKA LADYIRQQKS DPIQEIRDLA EITTLDRSKR NIIGYFEQKD SDNYRVFERV ANILHDDCAF LSAFGDVSKP ERYSGDNIIY KPPGHSAPDM VYLGAMTNFD VTYNWIQDKC VPLVREITFE NGEELTEEGL PFLILFHMKE DTESLEIFQN EVARQLISEK GTINFLHADC DKFRHPLLHI QKTPADCPVI AIDSFRHMYV FGDFKDVLIP GKLKQFVFDL HSGKLHREFH HGPDPTDTAP GEQAQDVASS PPESSFQKLA PSEYRYTLLR DRDEL|
|Grade & Purity||> 90% pure|
|Molecular Weight||48.0 kDa(415aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ERP44 is a novel endoplasmic reticulum chaperone involved in thiol-dependent retention of the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. The protein inhibits the calcium channel activity of ITPR1. This protein may have a role in the control of oxidative protein folding in the endoplasmic reticulum. Recombinant human ERP44 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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