FGF2 protein (30R-1206)

Purified recombinant Human FGF2 protein

Synonyms FGF-2, FGF 2, BFGF protein, fibroblast growth factor 2 protein, FGF2, FGF-2 protein, FGFB protein, FGF 2 protein, HBGF-2 protein, FGF2 protein, fibroblast growth factor 2
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast ) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) 5. Incubation time : 24 hr (after sample treatment) 6. Concentration range: 10 pg/ml - 1 ug/ml 7. Detection method : BrdU assay

Images

Coomassie Blue stained SDS-PAGE of FGF2 protein (30R-1206)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 143-288 amino acids: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 16.5 kDa (147aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 50mM Tris, pH 8.0.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is 0.1-1.5 ng/ml. Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Fibroblast growth factor 2 (FGF2) is a member of the FGF family that binds heparin and plays important roles in morphogenic, neurotrophic and angiogenic processes. FGF2 possess diverse biological functions, such as neuron differentiation, embryonic development and differentiation, modulation of angiogenesis, and wound healing. Recombinant human FGF2 was expressed in E. coli and purified by conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of FGF2 protein (30R-1206) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 50 ug
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