FITC antibody (biotin) (60R-FG003bt)

Goat polyclonal FITC antibody (biotin) conjugated

Synonyms Polyclonal FITC antibody, Anti-FITC antibody, Fluorescein antibody
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen FITC antibody (biotin) was raised in goat using fluorescein conjugated to goat IgG as the immunogen.

Images

Western blot analysis using FITC antibody (biotin) (60R-FG003bt)

Western Blot of Biotin Conjugated Fluorescein antibody. Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Fluorescein antibody at 1:1000 for 60 min at RT. Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 70 kDa, 70 kDa for BSA-FITC..

Specifications

Host Goat
Method of Purification FITC antibody (biotin) was purified by affinity chromatography.
Tag/Conjugate Biotin
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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Images

  • Western blot analysis using FITC antibody (biotin) (60R-FG003bt) | Western Blot of Biotin Conjugated Fluorescein antibody. Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Fluorescein antibody at 1:1000 for 60 min at RT. Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 70 kDa, 70 kDa for BSA-FITC..

Availability: In stock

Price: £396.62
Size: 1 mg
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