FKBP2 protein (30R-1506)

Purified recombinant Human FKBP2 protein

Synonyms FKBP 13 protein, FK506 binding protein 2 13kDa protein, FKBP 2, FKBP-2, FKBP13 protein, EC 5.2.1.8 protein, FKBP-13 protein, PPIase protein, Peptidyl prolyl cis trans isomerase protein, FKBP-2 protein, Rotamase., Peptidyl-prolyl cis-trans isomerase FKBP2 13 kDa FKBP protein, FK506 binding protein 2 (13kD) protein, Rapamycin binding protein protein, Proline isomerase protein, FKBP2, FKBP 2 protein, FKBP 2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant FKBP2 protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes

Images

Coomassie Blue stained SDS-PAGE of FKBP2 protein (30R-1506)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 22-142 amino acids: MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 13.4 kDa (122aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris buffer, pH 8.0, containing 10% glycerol and 1mM DTT.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 150 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH8.0 using chymotrypsin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance FKBP2 is peptidyl proline isomerase enzyme and a member of the immunophilin protein family. This protein is predominantly expressed in thymus and T cells and plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. FKBP2 is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Recombinant FKBP2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of FKBP2 protein (30R-1506) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 50 ug
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