G6PD protein (30R-1263)

Purified recombinant E.coli G6PD protein

Synonyms Glucose 6 phosphate 1 dehydrogenase protein, G6PD1 protein, zwf protein, GPD 6, Glucose-6-phosphate 1-dehydrogenase G6PD protein, Glucose-6-phosphate 1-dehydrogenase protein, G6PD, POS10 protein, GPD-6, GPD-6 protein, MET19 protein, GPD 6 protein, G6pdx protein, Zwf1p.
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 3ml reaction cocktail into a suitable container: The final concentrations are 50mM gylcylglycine, 2mM D-glucose 6-phosphate, 0.67mM beta-NADP, 10mM MgCl2. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 4ug of recombinant G6PD into reaction cocktail and mix immediately. 4. Record the increase at A340nm for 5 minutes.

Images

Coomassie Blue stained SDS-PAGE of G6PD protein (30R-1263)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-491 amino acids: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 55.7 kDa (491 aa), confirmed by MALDI-TOF.
Form & Buffer 50mM MES 6.0, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT, and 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphogluconolactone and simultaneously produces NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E. coli and purified by conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of G6PD protein (30R-1263) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £144.23
Size: 100 ug
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