GCN5L2 antibody (10R-1171)

Mouse monoclonal GCN5L2 antibody

Synonyms Monoclonal GCN5L2 antibody, Anti-GCN5L2 antibody, GCNL2 -5, GCN5 antibody, GCN5 antibody, GCN5L2, GCNL2 5, GCNL2 5 antibody, KAT2A antibody, K(lysine) acetyltransferase 2A antibody, general control of amino-acid synthesis 5-like 2 antibody, GCNL2 -5 antibody, PCAF-b antibody, hGCN5L2 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen GCN5L2 antibody was raised in mouse using recombinant human GCN5L2 (411-837aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of GCN5L2 antibody (10R-1171)

Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human GCN5L2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT3G13
Isotype IgG2a kappa
Source Anti-human GCN5L2 antibody, clone AT3G13, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GCN5L2 protein.
Method of Purification GCN5L2 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:500, WB: 1:250-1:500The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Each experiment should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance GCN5L2, as known as GCN5 and KAT2A is the use of immunoprecipitated GCN5L2 for acetylation to provide the additional components important for acetylation. GCN5L2 can be one of the potential enzymes involved in acetylation in vivo. GCN5L2 is expressed predominantly in the embryo and newborn and is essential for normal embryonic development. Therefore, GCN5L2 appears to be important for differentiation of embryonic-derived preadipocytes. On the one hand, loss of GCN5L2 leads to high incidence of apoptosis in the GCN5L2 mutants that begins before the onset of morphological abnormality.

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Images

  • Western Blot analysis of GCN5L2 antibody (10R-1171) | Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human GCN5L2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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Price: £212.33
Size: 100 ul
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