GCSF protein (30R-1052)

Purified recombinant Human GCSF protein

Synonyms CSF3 protein, Csfg protein, CSF beta protein, Granulocyte-colony stimulating factor Colony stimulating factor 3 (granulocyte) protein, MGC45931 protein, CSF 3 protein, , Granulocyte colony stimulating factor protein, Lenograstim protein, Filgrastim protein, Pluripoietin protein, GCSA protein, GCSF protein, Lenograstim protein, Macrophage granulocyte inducer 2 protein, G-CSF protein, G-CSF protein, MGI 2 protein, Granulocyte colony-stimulating factor protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of GCSF protein (30R-1052)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 18.8 kDa (175aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity The proliferative activity of rhG-CSF is tested in culture using murine bone marrow cells.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Human granulocyte colony-stimulating factor (hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of GCSF protein (30R-1052) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 50 ug
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