GCSF protein (30R-AG001)

Purified recombinant Human GCSF protein

Synonyms CSF-3 protein, Granulocyte Colony-Stimulating Factor protein, GM-CSF beta protein, MGI-1G protein, pluripoietin protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of GCSF protein corresponding to amino acids TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP.
Expression System E.coli
Grade & Purity > 98% pure
Molecular Weight 18.7 kDa
Form & Buffer Lyophilized from 5mM sodium citrate, pH 4.0. Reconstitute in water to a concentration of 0.1-1.0 mg/ml.

Usage & Assay Information

Bioactivity The ED50 as determined by the dose-dependent stimulation of the proliferation of Mouse M-NFS-60 cells is approximately 0.1 ng/ml, corresponding to a specific activity of approximately 1 x 10^7 units/mg.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and Mouse G-CSF are cross-species reactive.

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Availability: In stock

Price: £180.28
Size: 10 ug
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