Glycogenin 1 protein (T7 tag) (80R-1855)
Purified recombinant Human Glycogenin 1 protein (T7 tag)
|Synonyms||GYG1 protein, GYG 1., Glycogenin -1 protein, Glycogenin -1, Glycogenin1 protein, Glycogenin 1, Glycogenin 1, Glycogenin protein, Glycogenin 1 protein, GYG protein|
Coomassie Blue stained SDS-PAGE of Glycogenin 1 protein (T7 tag) (80R-1855)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-333 amino acids: MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMTDQAFVT LTTNDAYAKG ALVLGSSLKQ HRTTRRLVVL ATPQVSDSMR KVLETVFDEV IMVDVLDSGD SAHLTLMKRP ELGVTLTKLH CWSLTQYSKC VFMDADTLVL ANIDDLFDRE ELSAAPDPGW PDCFNSGVFV YQPSVETYNQ LLHLASEQGS FDGGDQGILN TFFSSWATTD IRKHLPFIYN LSSISIYSYL PAFKVFGASA KVVHFLGRVK PWNYTYDPKT KSVKSEAHDP NMTHPEFLIL WWNIFTTNVL PLLQQFGLVK DTCSYVNVED VSGAISHLSL GEIPAMAQPF VSSEERKERW EQGQADYMGA DSFDNIKRKL DTYLQ|
|Grade & Purity||> 90% pure|
|Molecular Weight||41.2 kDa (365aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Glycogenin 1 is an enzyme involved in glycogen biosynthesis. Glycogenin 1 is important for the function of self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Also, it plays a role in regulating glycogen metabolism and the attainment of maximal glycogen levels in skeletal muscle. Recombinant human Glycogenin 1, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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