GMCSF protein (30R-1055)

Purified recombinant Human GMCSF protein

Synonyms MGC131935 protein, GMCSF protein, Eosinophil Colony Stimulating Factor protein, CMCSF protein, Sargramostim protein, GM-CSF protein, Pluripoietin Alpha protein, Granulocyte Macrophage Colony Stimulating Factor protein, Colony-stimulating factor protein, , Colony Stimulating Factor 2 protein, CSF protein, Colony stimulating factor protein, CSF 2 protein, GM-CSF protein, CSF2 protein, CSF Alpha protein, Molgramostin protein, Granulocyte-macrophage colony-stimulating factor protein, MGC138897 protein, Granulocyte-macrophage colony stimulating factor Burst Promoting Activity protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: TF1 (human erythroleukemic cell). Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF Assay medium: 10% FBS RPMI 1640 without Gm-CSF Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation time: 24 hr (after sample treatment) Concentration range: 0.048 ng/ml - 25 ng/ml Detection method: MTT assay

Images

Coomassie Blue stained SDS-PAGE of GMCSF protein (30R-1055)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 14.6 kDa (128 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is < 0.18 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acid containing two disulfide bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of GMCSF protein (30R-1055) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £316.49
Size: 100 ug
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