Goat anti Mouse IgG (Fab'2) (PE) (43C-CJ0218)

Goat anti Mouse IgG secondary antibody (Fab'2) (PE)

Synonyms Goat anti Mouse Immunoglobulin G secondary antibody (Fab'2) (PE)
Specificity Murine IgG (Fab2)
Cross Reactivity To be determined by end-user
Applications FC, IF
Immunogen Goat anti-mouse IgG (Fab'2) (PE) was raised in goat using murine IgG F(ab’)2 fragment as the immunogen.

Images

Western blot analysis using Goat anti Mouse IgG (Fab'2) (PE) (43C-CJ0218)

Western blot of Phycoerythrin conjugated Goat F(ab’)2 Anti-Mouse IgG F(ab’)2 . Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. None. Phycoerythrin Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 kDa, 25 kDa for Mouse IgG F(ab’)2..

Specifications

Host Goat
Method of Purification Goat anti Mouse IgG (Fab'2) (PE) was purified by immunoaffinity chromatography, pepsin digestion and chromatographic separation.
Tag/Conjugate PE
Form & Buffer 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations FC: 1:100-1:250, IF: 1:100-1:250

Storage & Safety

Storage Store at 4 deg C. DO NOT FREEZE.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fab region, (fragment, antigen binding), is the binding region of the antibody.

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Images

  • Western blot analysis using Goat anti Mouse IgG (Fab'2) (PE) (43C-CJ0218) | Western blot of Phycoerythrin conjugated Goat F(ab’)2 Anti-Mouse IgG F(ab’)2 . Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. None. Phycoerythrin Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 kDa, 25 kDa for Mouse IgG F(ab’)2..

Availability: In stock

Price: £356.56
Size: 500 ug
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