Goat anti Rabbit IgG (41C-CB1143)

Goat anti Rabbit IgG secondary antibody

Synonyms Goat anti Rabbit Immunoglobulin G secondary antibody
Specificity Rabbit IgG
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Goat anti-rabbit IgG was raised in goat using rabbit IgG F(c) fragment as the immunogen.

Images

Western blot analysis using Goat anti Rabbit IgG (41C-CB1143)

Western Blot of Rabbit Fc antibody. Lane 1: Rabbit Fc. Lane 2: None. Load: 100 ng per lane. Rabbit Fc antibody at 1:1,000 for overnight at 4 deg C. DyLight649 Goat secondary antibody at 1:20,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Rabbit Fc. Other band(s): Rabbit Fc splice variants and isoforms.

Specifications

Host Goat
Method of Purification Goat anti Rabbit IgG was purified by affinity chromatography.
Form & Buffer 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3.
Concentration 2.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

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Images

  • Western blot analysis using Goat anti Rabbit IgG (41C-CB1143) | Western Blot of Rabbit Fc antibody. Lane 1: Rabbit Fc. Lane 2: None. Load: 100 ng per lane. Rabbit Fc antibody at 1:1,000 for overnight at 4 deg C. DyLight649 Goat secondary antibody at 1:20,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Rabbit Fc. Other band(s): Rabbit Fc splice variants and isoforms.

Availability: In stock

Price: £172.11
Size: 2 mg
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