Goat anti Rabbit IgG (H + L) (FITC) (43C-CB1109)
Goat anti Rabbit IgG (H + L) secondary antibody (FITC)
Overview
Overview
| Synonyms | Goat anti Rabbit Immunoglobulin G (H + L) secondary antibody (FITC) |
|---|---|
| Specificity | Rabbit IgG (H + L) |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, FC, IF |
| Immunogen | Goat anti-rabbit IgG (H+L) (FITC) was raised in goat using rabbit IgG whole molecule as the immunogen. |
Images
Western blot analysis using Goat anti Rabbit IgG (H + L) (FITC) (43C-CB1109)
Western Blot of Fluorescein conjugated Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. none. Fluorescein Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..
Goat anti Rabbit IgG (H + L) (FITC)
Western blot analysis using Goat anti Rabbit IgG (H + L) (FITC) (43C-CB1109)
Western Blot of Fluorescein conjugated Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. none. Fluorescein Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Goat anti Rabbit IgG (H + L) (FITC) was purified by affinity chromatography. |
| Tag/Conjugate | FITC |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | FC: 1:2,000-1:10,000, IF: 1:500-1:2,500 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. |
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